The Transmission Electron Microscopy laboratory (TEM) provides advanced expertise in cellular and material electron microscopy: from sample preparation, surface imaging and high-resolution ultrastructure of samples, to analysis of the acquired data.

Available methods

• Standard chemical sample preparation
• Cryofixation of samples by high pressure deep freezing
• Automatic freeze substitution
• Preparation of semthini and ultrathin sections
• Preparation of cryosections by Tokuyasu method
• Preparation of samples for immunolabeling
• Antigen detection and localization by immunogold labeling
• Negative staining for surface morphology

We analyze

• Proteins and macromolecular complexes
• Viruses, exosomes, liposomes
• Bacteria and yeast cells
• Molds and plants
• Adherent and suspension cells
• Tissues
• Organoids and small organisms
• Nanoparticles
• Materials

Instrumentation

• Electron microscope JEOL JEM 2100
• High pressure deep freeze Leica EM PACT2
• Freeze substitution Leica EM AFS2
• Ultramicrotome with cryo chambre Leica EM UC7

Contact: Romana Hadravová, ☎ 136