The flow cytometry laboratory is part of the IOCB core facility Biochemical Pharmacology. We provide state-of-the-art instrumentation in flow cytometry, theoretical and technical training, technical expertise, as well as cell sorting service on a regular base. Additionally, we offer consultancy on experimental design, sample preparation, data acquisition and analysis, as well as technical support and troubleshooting. The FCF currently runs two cell sorters (BD FACSAria IIu and CytoFLEX SRT) and two analyzers (BD LSR Fortessa and CytoFLEX LX), both equipped with High Throughput Sampler (HTS) module. We also have FlowJo analysis station. No training is required to run data analysis.


All sorts must be arranged and booked in advance with the operator. For sorting experiment, please fill Sort Request form   and send it to cytometry@uochb.cas.cz.


Location of the instruments

  • BD FACSAria IIu Cell Sorter: Building A - NW wing, 2nd floor, laboratory A.2.29
  • CytoFLEX SRT Cell Sorter: Building A - NW wing, 2nd floor, laboratory A.2.29
  • BD LSR Fortessa Flow Cytometer: Building C, 1st floor, laboratory C.1.32
  • CytoFLEX LX Flow Cytometer: Building C, 1st floor, laboratory C.1.32
  • FlowJo: Building A - NW wing, 2.np, laboratory A.2.30

For individual reservation, please use available Google calendars or contact the operators directly.

Contacts