2. Úvod
  3. Science
  4. Research Support
  5. Core Facilities
  6. Electromigration



Research-service group Electromigration methods provides following services:

Analysis

Qualitative and quantitative analysis of a wide spectra of ionogenic and electroneutral polar compounds: acids, bases, metal ions, amines, amino acids, peptides, proteins, nucleobases, nucleosides, nucleotides, fragments of nucleic acids, saccharides, steroids, etc.

The following capillary electromigration (CE) methods are available: zone electrophoresis (CZE) in a free solution (FSCE) or in sieving/gel media (CSE/CGE), isotachophoresis (CITP), isoelectric focusing (CIEF), affinity electrophoresis (ACE), electrokinetic chromatography (CEKC) and electrochromatography (CEC).

These methods can be used for determination of purity degree (electrophoretic or electrochromatographic homogeneity) of synthetic or isolated above compounds including determination of salts in their samples. These methods can be employed to monitor synthesis, preparation, isolation and purification of above compounds and the course of their chemical reactions, noncovalent interactions and enzymatic conversions. In addition, determination of selected compounds in complex (bio)matrices can be done by these methods.

Separation and Micropreparation

Separation of complex mixtures of ionogennic and electroneutral polar compounds, e.g. peptide and protein profiling in biomatrices, peptide mapping of proteins, restriction mapping of nucleic acids, etc. Separation of isomers (positional, geometric, optical – enantiomers and diastereomers) of the above compounds in analytical or micropreparative scale. Isolation of above compounds in a microscale (100 ng – 2 µg).

Physico-chemical Characterization

Determination of the following physico-chemical quantities of ionogenic compounds: effective electrophoretic mobility, actual and limiting (absolute) ionic mobilities, effective charge, isoelectric point, hydrodynamic radius, relative molecular mass, diffusion coefficient, acididy constant (pKa), binding (association/dissociation, stability) constant of complexes (Kb, KD, Kst). From the determined binding/dissociation constant of complex and its temperature dependence, the changes of Gibbs energy, enthalpy and entropy during the complex formation can be estimated.

Instruments

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