Currently, we provide antiviral screening of compounds prepared in the institute against viruses listed below. For active compounds, we provide also cytotoxicity measurements in cell line used for the particular antiviral screening.

Ongoing screening of compounds continuously added to the IOCB compound library

1. RNA viruses
   • ssRNA-RT
     - Human immunodeficiency virus type 1 in MT-4 cells
   • +ssRNA
     - Human coxsackie B3 virus in HeLa cell
     - Dengue type 2 virus in Vero cells
     - SARS-CoV-2 in Vero E6 cells
   • –ssRNA
     - Influenza A H1N1 in MDCK cells
2. DNA viruses
   • Herpes simplex type 1 virus in Vero cells

Screening of whole IOCB compound library (year of completion)

• African swine fever virus (2018)
• Zika virus (2019)
• SARS-CoV-2 (2020-2021)
• Screening for HIV latency reversal agents (2019)

Plan of screening for year 2023: Respiratory syncytial virus (RSV), monkeypox virus

Other viruses available in our virus bank: Hepatitis B virus, Aichivirus, Dengue type 1, 3 , and 4 virus, Hepatitis D virus, Herpes simplex type 2 virus, Human adenovirus B type 3, Human immunodeficiency virus type 2, Influenza A H3N2 virus, Influenza B virus, Reovirus 3 strain Dearing, Rotavirus A, murine norovirus, human coronavirus 229E and OC43, Rift Valley fever virus, respiratory syncytial virus, monkeypox virus

Available equipment

• VICTOR X5 plate reader includes luminescence, fluorescence (both top and bottom reading), UV-absorbance (UV-VIS)
• Guava easyCyte 6HT-2L flow cytometer (96-well)
• fluorescence microscope Olympus IX71 with camera
• Optima Max-XP Biosafe Ultracentrifuge
• cell sorter Sony MA900

Instructions for compounds screening request

• Preferentially the requester will add their compounds into IOCB compound library. This procedure will guarantee the testing of the compounds against all currently used and future viruses. For special requests, contact Jan Weber.
• In addition, we are able to perform custom-made experiments involving BSL3 and BSL2 pathogens (virus isolation and culturing, DNA and RNA isolation from viruses, fluorescence immunochemistry techniques, ELISA, western blots, reverse transcriptase activity assays, FACS of virus-infected cells, etc.).

Did you not find your virus of interest? We welcome any suggestions, comments and questions. Are you interested in doing your own virus experiments? We offer safety training and basic training in virus-related cell culture techniques.