Equipment

UHPLC Vanquish Thermo Scientific & PAL-RTC

The liquid chromatograph Vanquish UHPLC Thermo Fisher includes quarternary pump (VC-P20-A-01), autosampler (VC-A13-A) and double detection of Diode Array Detector (VC-D11-A-01) and Charged Aerosol Detector (VH-D20-A). The DAD works in wavelenght range od 190-800nm. The CAD allows to detect semi- and non-volatile compounds even if there is lack of chromophore. The main advantage of CAD is also wide linear dynamic range and sub-nanogram sensitivity. We perform mostly RP-HPLC and also HILIC separations.

The PAL-RTC system is used for precise automated sample preparation. The robotic arm provides three syringes of different volume (25, 250 and 1000µl) and it is possible to work with 2 ml HPLC compatible vials. The main advantage is minimal sample and reagent consumption.