Samoobslužný servis na 400-MHz NMR spektrometrech

The self-service NMR facility is available to be used by all scientific personnel at IOCB. In order to begin measuring your own NMR experiments, a short training session must be undertaken first.

Please, contact us at nmr-400@uochb.cas.cz to make an appointment.

• Self-service guidelines  
• Check the queue status at Bruker Avance IIIT HD 400 MHz ("OLD400")
• Check the queue status at Bruker Avance IIIT HD 400 MHz with cryoprobe ("PRODIGY")
• Accessing you data

NMR spectrometers

• Bruker Avance IIIT HD 400 MHz ("OLD400")
• Bruker Avance IIIT HD 400 MHz with cryoprobe ("PRODIGY")

FAQ

1. Why we cannot measure NOE-type experiments?

   Our goal is to keep the night queue shorter than one night as often as possible. Unfortunately NOESY or ROESY experiments are usually quite time-consuming. To be meaningful, these experiments would require on the 400 MHz spectrometers several hours of the spectrometer time that would lead to significantly longer night queues. An alternative to the 2D NOE experiments is the measurement of 1D differential NOE spectrum. However, this experiment requires a careful experimental setup for each sample, which is not possible in the self-service automation mode.

   If you need any NOE-type experiment, we will be happy to measure it for you on other spectrometers of the NMR Spectroscopy group.
   
   

2. How should we describe the 400MHz spectrometers in methodological parts of publications?

   Please edit the following text according to your needs:
   The NMR spectra were measured on a 400 MHz Bruker AVANCE III spectrometer (¹H at 400 MHz) or 401 MHz (PRODIGY), ¹³C at 101 MHz, ¹⁹F at 377 MHz, ³¹P at 162 MHz) equipped with a liquid-nitrogen cryoprobe (PRODIGY). The spectra were referenced to the solvent signals δ(¹H) = 7.26 ppm (CHCl₃), δ(¹H) = 3.31 ppm (CHD₂OD), δ(¹³C) = 77.0 ppm, (CDCl₃), δ(¹³C) = 49.0 ppm (CD₃OD), ...
   
   

3. What is the number of scans in the experiments?

   Experiment	PRODIGY
   1H	16
   13C_long, APT_long	528
   31Pdec	16
   31Pnodec	32
   19F_nodec	16
   COSY	1 scan, 128 increments
   HSQC	2 scans, 256 increments
   HSQC_NUS	4 scans, 256 increments,25% sampling
   HMBC	2 scans, 256 increments

   
   
   
4. What is the recommended minimal sample amount for the experiments?
   For a decent organic molecule (Mr < 500), proton spectra can be measured with a few milligrams of the compound, but carbon spectra require at least 10 mg.
   
   
5. Where can I get NMR tubes and deuterated solvents?
   NMR Tubes are available in the storage of consumable supplies in room B.01.12 and deuterated solvents can be retrieved from chemical storage in room B.1.07. Make sure to come with a signed issue slip.